In this project, a new method for imaging tissues will be developed using novel carbon nanotube based nanoparticles that emit a strong Raman signal enabling the simultaneous detection of 15 biomarkers. The validation of this method will be accomplished by comparing results to classic immunohistochemistry (IHC) and 6 -plex immuno-fluorescence imaging of tissues. 15 Raman-labels will be developed to be specific to immunogenic biomarkers and to breast cancer biomarkers. The development of the method will offer a tool to researchers to advance immunotherapy in breast cancer.